Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi

While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting purifying target from cellular system is also required to characterize enzyme. This is, particular, necessary before developing industrial-scale production. In present study, partially purified α-amylase was obtained strains Pseudogymnoascus sp. Antarctic Arctic locations. Partially α-amylases these polar fungi exhibited very similar characteristics, including being active at 15 °C, although having a small difference optimum pH. Both taxa good candidates potential application cold-active enzymes biotechnological industries, further purification characterization steps now required. The attractive terms industrial development because they lower temperatures acidic pH, thus potentially creating energy cost savings. Furthermore, prevent production maltulose, which an undesirable by-product often formed under alkaline conditions. Psychrophilic amylases investigated study could provide valuable future contribution applications.